Al Del Greco kicked a 36-yard field goal in overtime as the Titans rallied in their home opener.
The victory kept the AFC champions from going into the bye week without a victory. It also kept them undefeated in Adelphia Coliseum, tying the NFL record held by the 1971 Dallas Cowboys with nine victories to christen a stadium.
"We take a lot of pride in playing here at this coliseum and to win like this will probably add that much more hype to the mystique of this coliseum," Titans safety Marcus Robertson said.
The Chiefs had a chance to win with the score tied at 14 late in regulation.
A pass interference call against Denard Walker, who missed the opener under a one-game suspension by the NFL, moved the Chiefs to the Titans 43. Elvis Grbac then found Tony Gonzalez with a 9-yarder to the 34, seemingly setting up kicker Pete Stoyanovich to win it.
He never got the chance. Chiefs receiver Derrick Alexander was penalized for pass interference and Grbac picked up a penalty for intentional grounding, pushing the Chiefs to their 45. They wound up punting and heading into overtime.
"We had a great opportunity," Grbac said. "We let it slip right through our hands."
The Titans' Neil O'Donnell, who came in when quarterback Steve McNair bruised his chest, threw 8 yards to Yancey Thigpen with 50 seconds left to tie at 14.