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Anti-abortion activists protested at more than a dozen Planned Parenthood facilities across Florida on Saturday.
State health officials recently inspected 16 Planned Parenthood facilities and said three were performing unlicensed second-trimester abortions.
Planned Parenthood disputed the finding, saying the state changed its definition of how they measure first-trimester.
The investigation ordered by Republican Gov. Rick Scott mirrors similar probes in other states after stealthily recorded videos showed Planned Parenthood officials discussing how they provide aborted fetal organs for research. The organization has said it does not profit from tissue donations and that those programs exist in only a handful of states.
Abortion opponents say the videos show the organization is illegally harvesting and selling organs.
Investigations in Massachusetts, Georgia, Indiana and South Dakota found no evidence of wrongdoing.